The Chosen procedure for membranes synthesis is briefly described as following:
The problem that the invention solves is to use a dialysis membrane that can treat both medical conditions at the same time. The procedure will have a major impact on the quality of life of patients suffering from both medical conditions. The dialysis membrane with the ability of controlled release of doxorubicin for the simultaneous treatment of chronic renal dysfunction and liver cancer, according to the invention, significantly improves the medical procedures for the simultaneous treatment of the two diseases.
The process of making the dialysis membrane with controlled release capacity of doxorubicin for the simultaneous treatment of chronic renal dysfunction and liver cancer, according to the invention, consists of:
- Making a solution in N, N-dimethylformamide of α-cyclodextrin (5% by mass) aminopropyl triethoxy silane (2% by mass), doxorubicin (2% by mass) and sodium hydroxide (0.1% by mass);
-For the synthesis of supramolecular architectures, the solution is kept at 60 °C for 12 hours, under mechanical stirring with a magnetic stirrer at 400 rpm;
-For the synthesis of supramolecular architectures for stimulated release of doxorubicin, after the completion of the first reaction, monoclonal antibody for α-fetoprotein (0.06% by mass) is added to the reaction mass, the temperature is lowered to 40 °C and the mechanical agitation is maintained for 40 hours, at a speed of 200 rpm;
-After the synthesis of the supramolecular architectures, they are precipitated from the reaction mass with acetone and filtered on sterile Nylon membranes with a porosity of 0.20 mm, the obtained powder being washed three times with deionized water and dried;
-For the synthesis of polysulfone membranes, a polymer solution in N,N-dimethylformamide (12% by mass) will be prepared by mechanical stirring at 60 °C for 12 hours; the membranes are obtained by depositing a film of polymer solution on a glass support at a thickness of 150 mm and coagulate in a water:ethanol 1:1 mixture;
-Plasma modification of the polysulfone membrane for dialysis, for the induction and generation on the surface of functional groups – CHO;
-Immobilization on the membrane surface of supramolecular architectures with doxorubicin-stimulated release capacity with the formation of Schiff bases through the reaction between the – CHO group on the membrane and the – NH2 groups coming from APTES.
The invention presents the following advantages:-The process of obtaining the double-role membranes is non-toxic;-The dual-role membrane can simultaneously treat two major medical conditions;-The dual-role membrane can lead to a significant increase in the quality of life of patients suffering from chronic renal dysfunction and liver cancer;-The impact on the general condition of the body generated by the treatment with doxorubicin is significantly diminished because the amount of cytostatic drug that is discharged into the body is directly proportional to the concentration of α-fetoprotein in the blood (which in turn is directly proportional to the size and evolution of the tumor) ;-The economic impact generated by the invention will also be a major one, by significantly reducing the costs of hospitalization and the adjacent expenses associated with it (e.g. food, medical staff salaries, utilities), as well as by reducing the pressure on the oncological facilities in the hospital environment.
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